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Sol-Gel Synthesis of NiO nanoparticle using Excess CO2 Acidification(ECA)

A novel sol–gel-based Excess CO₂ Acidification (ECA) method was proposed to synthesize uniform metal oxide nanoparticles with consistent size, morphology, and mesoporosity. In this method, CO₂ was over-sparged into a buffered KOH solution to generate an acidic bicarbonate buffer, followed by addition of metal precursor solutions and controlled reduction of CO₂ concentration established a proton concentration gradient. This approach ensures homogeneous sol–gel reactions, prevents loc...

— HongKi, Yoon 2026-01-16 00:00:00

Engineering Avidin-Functionalized Gold Nanoparticles for LIGMA: A Cationic Isotachophoresis-Driven Lateral Flow Assay

Lateral flow assays (LFAs) are widely used for rapid, point-of-care diagnostics but struggle with sensitivity in part due to passive migration and interference from high-abundance serum proteins. Here, we introduce the Lateral Isotachophoresis-Guided Migration Analysis (LIGMA) assay, a novel diagnostic platform that leverages cationic isotachophoresis (+ITP) to enhance biomolecular separation and detection in LFAs. Using avidin-functionalized gold nanoparticles (AV-S-GNPs) as positively charg...

— Wen-Ji, Dong 2026-01-16 00:00:00

Dual Determination of Nitrogen Content and Nitration Site Distribution in Nitrocellulose via HSQC NMR

We report the successful NMR characterisation of cellulose trinitrate (CTN) and preliminary findings for cellulose dinitrate (CDN). We also present initial work towards a 15-second reagent-free methodology for the estimation of degree of nitration (DoN) in mixed nitrocellulose samples, using grouped integrals from 1H NMR. This offers a pathway to resolve polymolecularity challenges in nitro-polysaccharide analysis, supporting the development of high-performance nitrocellulose membranes for e...

— James, Boxall-Clasby 2026-01-16 00:00:00

Digitized dataset of aqueous dissociation constants

The acid dissociation constant (pKa) quantifies the acidity of a compound, which is crucial for applications including drug design, environmental fate studies, and chemical synthesis. However, high-quality open-source digital pKa datasets are scarce, which limits the ability for researchers to search for properties of individual compounds, while also limiting the potential of data-driven predictive models. In this work, we release the IUPAC Digitized pKa Dataset, a digital version of a critic...

— William, Green 2026-01-16 00:00:00

Repurposing Dna-Binding Proteins as Molecular Actuators in Synthetic Systems Through Proximity-Driven DNA Reactions

DNA-binding proteins (DBPs) play central roles in gene regulation by recognizing specific DNA sequences, yet their function in synthetic molecular systems is typically limited to transcriptional control or sequence-specific binding. Here we describe a proximity-driven molecular framework that couples DBP–DNA recognition to DNA strand displacement reactions, enabling DNA-binding proteins to act as sequence-selective inputs for synthetic nucleic acid reaction pathways without modification of ...

— Sofia, Marzari 2026-01-16 00:00:00

Synthesis of microbial-derived octadecanoids

The gut microbiome is increasingly being shown to exert multiple biological effects upon the host. This activity can occur via the production of microbial-derived metabolites. Gut bacteria have been demonstrated to convert dietary polyunsaturated fatty acids (PUFAs) into oxygenated metabolites called oxylipins, which can exert potent lipid mediator functions. The products derived from C-18 fatty acids are collectively known as octadecanoids and have been shown to associate with multiple disor...

— Mats, Hamberg 2026-01-16 00:00:00

A direct LC–MS/MS method for simultaneous quantitation of bisphenol S, propylparaben, monobutyl phthalate, and their metabolites in human urine

Accurate biomonitoring of non-persistent endocrine-disrupting chemicals (EDCs) requires robust quantitative methods for reliable human exposure and risk assessment. Building on previous work showing that direct measurement of bisphenol A and its conjugated metabolites in human urine reveals substantial underestimation in commonly used indirect, hydrolysis-based approaches, this study addresses the broader need for direct assays of other short-lived EDCs. Here, a direct liquid chromatography...

— Patricia, Hunt 2026-01-15 00:00:00

Discovery of a Dual-Target DNAzyme for Clostridioides difficile and Listeria monocytogenes Diagnostics

Rapid and reliable detection of Clostridioides difficile is essential for clinical diagnosis and infection control. Building on our group’s prior development of RNA-cleaving fluorogenic DNAzymes (RFDs) for bacterial recognition, we report RFD-CD3, a fluorogenic DNAzyme with the highest catalytic efficiency reported to date for C. difficile-responsive DNAzymes, achieving a detection limit of 10³ CFU/mL and a catalytic rate constant (kobs) of 0.16 min⁻¹ for its engineered trans-acting var...

— Yingfu, Li 2026-01-15 00:00:00

Framework of Copolymer gel Dynamics and Ligand Interaction Factors, Objective-free exploration, and CVT-MAPElites

Discovery-oriented research is a fundamental pursuit in chemical and materials science, especially when objective-free or purpose-ambiguous exploration can yield unexpected novel compounds or materials. Recently, data-driven objective-free exploration methods have emerged to support such discovery in materials science. However, these methods have mostly been limited to relatively simple systems (e.g., chemical composition), and extending them to real-world multiscale, multi-component material...

— Wenrui, Zhu 2026-01-14 00:00:00

Clarifying Stereochemical Outcomes in Radical-Initiated Vinyl Cyclopropane Cycloadditions within the Beckwith-Houk Framework

The (3+2) cycloaddition reaction is a widely utilized strategy in organic synthesis for constructing cyclopentane frameworks, a structural motif prevalent in many bioactive compounds and pharmaceutical applications. Over the past decades, numerous catalytic radical cascade reactions orchestrated by various types of open-shell catalysts have been reported. However, discrepancies in the relative configurations of cyclopentanes obtained using closely related methodologies suggest that some stere...

— Robert, Paton 2026-01-14 00:00:00

Native Charge Detection Mass Spectrometry of Kilobase-Scale Messenger RNAs

Charge detection mass spectrometry (CDMS) enables direct measurement of mass and charge for individual ions and overcomes fundamental limitations of conventional electrospray mass spectrometry for large, heterogeneous biomolecules. Messenger RNAs (mRNAs) represent a particularly challenging analytical target, as transcripts often span thousands of nucleotides, exhibit substantial heterogeneity, and form higher-order oligomers that are difficult to assess using existing workflows. Here, we opt...

— Weijing, Liu 2026-01-13 00:00:00

Nanopore DNA sequencing with a sub-nm stop-and-go mechanical positioning system and current blockades or fluorescent tags

DNA sequencing using nanopores and a mechanical positioning system with 0.1-0.15 nm movement precision and ~1 μs movement times is proposed. A single strand of DNA (ssDNA) is attached to a homonucleotide header immobilized on a glass slide mounted on a platform in the cis chamber of an electrolytic cell (e-cell) with a nanopore. The header-strand is moved into the trans chamber and retracted mechanically one nucleotide at a time. Two approaches to sequencing are described. In the first the b...

— G, Sampath 2026-01-13 00:00:00

Separation and preconcentration of biomolecules using ion concentration polarization in microchannels

Ion concentration polarization (ICP) is an effective technology for preconcentrating dilute charged species in microfluidic systems for various applications across chemistry and biology. In this work, we concurrently separate and preconcentrate the biomolecules of interest from mixed samples in microchannels based on their differences in electrophoretic mobilities. By numerically solving the coupled governing equations of the Nernst-Planck, Poisson, and Navier-Stokes, we systematically study ...

— Van-Sang, Pham 2026-01-13 00:00:00

Let’s Get Cyclic – Influence of Head-to-Tail Cyclisation of Lanmodulin-inspired peptides on Lanthanide Affinity and Structure

Over the recent years lanthanide-binding proteins such as lanmodulin, a naturally lanthanide-binding EF-hand protein from Methylorubrum extorquens AM1, as well as protein-inspired lanthanide-binding peptides have become of increasing interest for the development of separation and recycling methods for rare earth elements. For the first time, a set of head-to-tail cyclised lanmodulin-inspired peptides and their complexation behaviour towards lanthanides was comprehensively examined in aqueous ...

— Lena J. , Daumann 2026-01-13 00:00:00

Oxyanion Isotopologue Analysis of Natural Samples by Electrospray-Orbitrap: Method, Validation and Clumped Isotopes

Traditional gas-source, sector-field isotope ratio mass spectrometers are not optimally designed to analyse non-volatile molecular solutes such as oxyanions or organics. Tedious preparation steps – often requiring micromole-scale samples – break chemical bonds, meaning only elemental isotope ratios can be extracted. Recent advances in mass spectrometry point towards a novel isotopic technique: soft electrospray ionisation coupled to a high-resolution Orbitrap mass spectrometer (ESI-Orbitr...

— Joël, Savarino 2026-01-12 00:00:00

Quantitative Host Cell Protein Analysis of Antibody-Based Protein Therapeutics using the Orbitrap Astral Mass Spectrometer

Host cell proteins (HCPs) are endogenous proteins generated in cellular production systems alongside the biotherapeutic of interest. Removal of HCPs is crucial as they can be detrimental to product efficacy and patient safety. Due to its ability to determine individual HCP concentrations, liquid chromatography tandem mass spectrometry is increasingly utilised as an orthogonal method to ELISA for HCP monitoring. For protein biotherapeutics like monoclonal antibodies, their dynamic range makes ...

— Nicolaie Eugen, Damoc 2026-01-12 00:00:00

A Reversible ATR-FTIR Biosensor with Single Residue Sensitivity

Infrared spectroscopy provides label-free access to protein structure information with high sensitivity. Challenges in infrared spectroscopy include artifacts arising from temperature changes, baseline drifts and the strong water absorption, especially in difference spectroscopy, where signals are orders of magnitude smaller than the total absorbance. Previously, we introduced an ATR-based immuno-infrared sensor (iRS) based on sophisticated surface chemistry. This provides a stable platform f...

— Klaus, Gerwert 2026-01-12 00:00:00

High performance interface for boosting ion transmission in native mass spectrometry and beyond.

Electrospray ionization (ESI)-based native mass spectrometry (MS) has been extensively used to study non-covalent protein complexes and their structural aspects in the gas phase. As such it has a stable place in the portfolio of modern structural biology and biopharmaceutical analysis techniques. It is also promising as an approach to deliver pro-teins and their assemblies into the gas phase for single particle experiments at advanced light sources such as free electron lasers and synchrotron...

— Charlotte, Uetrecht 2026-01-10 00:00:00

Overcoming Sensitivity Barrier in 13C NMR Using Frequency–Time Dual-Domain GANs: Quantitative Reaction Monitoring at Natural Abundance

Low sensitivity has long limited the use of heteronuclei such as 13C and 15N NMR for quantitative chemical measurements, confining reaction monitoring largely to proton-detected experiments. Although highly sensitive, proton NMR often becomes unreliable due to severe signal overlap. Here we show that machine-learning-enabled heteronuclear NMR overcomes this limitation, enabling accurate, time-resolved reaction monitoring using natural-abundance 13C nuclei without isotopic enrichment or hardwa...

— Subhankar, Mishra 2026-01-10 00:00:00

On Repeatability of Electrochemical Zinc Nucleation and Growth from Aqueous Zinc Sulfate Electrolytes

Electrodeposition of zinc is critical to enabling rechargeable zinc batteries. Historically, zinc batteries have employed zinc foil or powder zinc anodes, and the anode research has focused on improving the reversibility of zinc electrodeposition through a combination of material modifications and electrical controls. With breakthroughs in anode-free lithium batteries, an appealing possibility is to leverage the anode-free configuration. The very first step in such an operation is nucleation ...

— Trevor, Sando 2026-01-09 00:00:00

DynaSpec: Metadynamics and Raman Spectroscopy for Glycan Structure–Spectrum Mapping

Raman spectroscopy offers label-free access to molecular vibrations across biological systems of varying complexities, from small molecules to flexible biomolecular oligomers and even whole cells. While Raman spectra of small molecules often permit direct structural interpretation, intermediate-sized systems—such as glycans, short nucleic acids, and peptides—exhibit higher compositional and conformational heterogeneity that obscures structure–spectrum relationships. We introduce DynaSpe...

— Jennifer, Dionne 2026-01-09 00:00:00

Differential Coats-Redfern Framework for Conversion-Resolved Temperature-Programmed Kinetics

Temperature-programmed measurements such as thermogravimetry (TG) are routinely interpreted with Coasts-Redfern (CR) or isoconversional methods. Classical CR provides model-dependent activation energies (Ea) and pre-exponential factors (A) from a single heating ramp but relies on a global straight-line fit of ln[g(α)/T2] versus 1/T, which does not provide a rigorous differential definition of Ea and averages over curvature, obscuring local kinetic information since 1964. In this study, we re...

— Young Soo, Kang 2026-01-08 00:00:00

Characterizing Nanostructured Films Using Phase Sensitive Vibrational Sum Frequency Spectroscopy

Nanoparticle films are ubiquitous thermal and electrocatalysts, yet their \textit{operando} characterization remains challenging. Vibrational sum-frequency generation (vSFG) spectroscopy offers unique advantages due to its high sensitivity and surface specificity, but its application to systems with such intermediate length scale disorder, particularly with phase-resolved detection, has been challenging. In this study, we describe an approach to phase-resolved vSFG spectroscopy of nanoparti...

— Gerd, Bacher 2026-01-08 00:00:00

Nanoscale morphometric visualization of the endoplasmic reticulum under stress using a bright zinc complex

The endoplasmic reticulum (ER) undergoes dynamic morphological changes during cellular stress and disease, yet specific staining and nanoscale observation remain challenging. We report a highly bright, biocompatible, and nontoxic fluorescent Zn-complex for super-resolution imaging of the ER. Super-resolution radial fluctuation microscopy (SRRF) resolves the smallest tubular diameter of ER, 110 nm, and quantifies stress-induced morphological remodeling.

— Shilpa , Chandra 2026-01-08 00:00:00

O-glycosylation affects proteolysis of the native tear peptidome

immune response and participating in antimicrobial activity. The biological function of these native peptides is governed by their biochemical properties including peptide length, charge, and hydrophobicity. Accordingly, the production of native peptides within the tear film is tightly regulated through a complex interplay between circulating proteases and the proteolytic susceptibility of the protein substrate. Though glycosylation has been shown to regulate the proteolysis of specific prote...

— Keira, Mahoney 2026-01-07 00:00:00

Memory effects in active droplets

Marangoni-driven self-propelled droplets have attracted considerable attention as a platform to study active soft matter and collective behavior. The speed of active drops is generally assumed to be determined by instantaneous diameter and solubilization rate, which together dictate the spontaneously generated interfacial tension gradients across the drop surface and induce propulsion; the history of the droplet has not before been considered. Here, we report that active droplets can exhibit ...

— Bryan, Kaehr 2026-01-07 00:00:00

Return on Experiment: Information-per-Cost Metrics for Self-Driving Chemical Laboratories

Self-driving laboratories (SDLs) integrate artificial intelligence with laboratory automation to accelerate chemical optimization through iterative experiment design, execution, and analysis. Current evaluation practices emphasize outcome-centric metrics such as best-achieved yield, number of experiments to reach a target, and Pareto-front advancement. However, these summaries do not explicitly quantify learning efficiency—how much model-relevant information is gained per unit resource—no...

— Samrat, Chakraborty 2026-01-06 00:00:00

Assessment of Protein Conformation via Diazirine-Promoted Oxidation

Photo-affinity labeling (PAL) utilizes photoreactive molecules to derivatize proteins in a solvent-accessible depend-ent manner. The site and frequency of the products of this reaction can be used to garner insights into the confor-mation of the protein. In this work, we document and characterize a novel oxidation process instigated by UV irradia-tion of aromatic diazirines. Diazirine is an increasingly utilized reagent for probing protein conformations, and this product has yet to be documen...

— Salem, Neufeld 2026-01-06 00:00:00

Fate of organic contaminants in electrochemical nitrogen recovery from urine

This study compared the fate of pharmaceuticals and disinfection byproducts across three electrochemical nitrogen recovery processes treating urine: electrochemical stripping (ECS), electrodialysis (ED), and bipolar electrodialysis (BPED). ECS achieved greater TAN recovery efficiencies than ED and BPED and was the only process that recovered a urine-derived ammonium sulfate product rather than a mixed concentrate (containing TAN, sodium, and potassium). Enrichment and removal ratios based on...

— William, Tarpeh 2026-01-06 00:00:00

Native multi-dimensional (SEC/CZE/SID) protein complex identification and topology characterization (Native MudPIT)

Here, we demonstrate pilot development of native multi-dimensional protein complex identification and topology characterization (Native MudPIT) in complex biological samples via coupling native size exclusion chromatography (SEC), online native capillary electrophoresis-mass spectrometry (nCE-MS), surface-induced dissociation (SID), and multi-level proteomics. With semi-manual data interpretation because optimized algorithms do not yet exist for this approach, the Native MudPIT approach enabl...

— Fei, Fang 2026-01-06 00:00:00

Analysis of small microparticles from snow in Park City, Utah (USA): How much of it is plastic?

Fresh snow samples (50mL) were collected from 3 sites in the ski area and from 2 sites in the ski resort, all exposed to different levels of traffic of people. We performed epifluorescence imaging to detect small microplastics that are comprised between 1-100microns in size. Small microparticles concentrations ranged from 1,000-4,000/50mL in the ski resort, whereas these numbers ranged from 30 to 3,000/50mL in the ski area. Against expectations, the higher and most remote site contained the g...

— Avery, Dougherty 2026-01-06 00:00:00

Molecular Selectivity of Damping-Induced Spectral Amplification

Environmental damping in molecular spectroscopy is commonly treated as a phenomenological source of decoherence and spectral broadening, leading to a monotonic reduction of spectral intensity. Here we show that this assumption is not generally valid. Using a vibronically resolved response-function framework, we demonstrate that for certain molecular vibronic structures, intermediate damping can enhance observable spectral intensity through redistribution of vibronic interference, without any ...

— Yu-Yang, Chen 2026-01-05 00:00:00

Theoretical Framework for Absolute Quantification in Digital Immunoassays and Advancements in Microfluidic-Free Droplet-Based Digital Immunoassay Methodologies

Current single-molecule immunoassays primarily rely on Poisson distribution models, which have limitations in achieving absolute quantification of target proteins. To address this, we developed a novel mathematical model that treats the capture of target proteins by magnetic beads as a discrete Poisson process, while the subsequent washing and secondary sampling steps are modeled using a hypergeometric distribution. Based on this combined distribution model, we constructed a new theoretical f...

— Qingxin, Song 2026-01-05 00:00:00

First evidence of microplastics in bottled water sold in Mexico

Given that Mexico is one of the world's leading consumers of bottled water per capita, this study provides the first evidence of microplastic contamination across ten widely consumed commercial brands in the country. A total of 2,358 MP particles were detected with an average concentration of 39.3 ± 14.6 particles/L. Statistical analysis (ANOVA, p = 0.0008) confirmed significant differences in MP levels between brands, likely attributed to variations in the production process and packaging q...

— José Luis , Cabellos-Quiroz 2026-01-05 00:00:00

Functional Group Identification from Infrared Spectra via a Dual-Head Convolutional Network

Infrared (IR) spectroscopy is widely used for molecular characterization, yet automated functional group identification remains challenging due to spectral overlap, weak absorptions, and context-dependent vibrational features. In this work, we present a dual-head convolutional neural network for multi-label functional group identification directly from single infrared spectra. The proposed architecture captures complementary local and global spectral information through parallel convolutional...

— Yitian, Kong 2026-01-05 00:00:00

Tailoring TiO2 Morphology and Surface Chemistry for Optimized Photocatalytic Activity in rGO Hybrids

TiO2–reduced graphene oxide (rGO) hybrids were investigated in this study to elucidate how TiO2 morphology and surface chemistry govern charge-transfer pathways and, ultimately, reaction selectivity. Three anatase TiO2 nanostructures were compared: bipyramids predominantly exposing {101} facets (bipy) and two nanosheet-like samples enriched in {001} facets, either fluorinated (n-sh) or thermally defluorinated and {101}-enriched (n-sh_873K). A constant rGO loading (2 wt.%) was introduced via...

— Fabrizio, Sordello 2026-01-04 00:00:00

Cellulose Coating Altered the Electro-Chemo-Mechanical Evolution of Sodium Thioantimonate Electrolyte in Solid-state Sodium Batteries: An Operando Raman Study

All-solid-state batteries (ASSBs) attracted increasing attention due to their improved safety and energy densities; yet electrolyte decomposition and subsequent contact loss limited the interfacial stability of ASSBs. Herein, we report an operando Raman characterization that provides high voltage, time, and spatial resolutions, which enables simultaneous analysis of interfacial decomposition mechanism and morphological evolution. Using Na3SbS4 electrolyte (NSS) and its carboxymethyl-cellulose...

— Shihong, Xu 2026-01-04 00:00:00

Leonardo under cover

The study of artwork is an interesting field that has undergone a major turn the last decades as new scientific analysis methods have been introduced and is becoming a significant part of the investigations. In this study we have employed Raman spectroscopy to analyse the pigments in an old painting of the Roman Emperor Domitian. The painting is of special interest since its creation is historically attributed to Tiziano Vecellio, more known as Titian, but it has recently been found to contai...

— Lars, Helldahl 2026-01-04 00:00:00

Deep Eutectic Solvent (DES)–Based Gels: Structure, Properties, and Emerging Pharmaceutical Applications

Deep eutectic solvents (DES) have emerged as highly versatile, green, and tunable solvents that exhibit remarkable hydrogen-bonding capacity and excellent biocompatibility. When incorporated into polymeric networks, they form DES-based gels—hybrid semi-solid systems that merge the structural integrity of gels with the functional advantages of DES. These materials demonstrate exceptional potential in pharmaceutical sciences by offering improved solubility for poorly soluble drugs, enhanced d...

— Harveen , Kaur 2026-01-03 00:00:00

Reviving Algebraic Structure Approaches for Hidden Subgroup Variants and Novel Quantum Speedups in the NISQ Era

The Hidden Subgroup Problem (HSP) unifies several landmark quantum algorithms, yet systematic exploration of its variants and modern applications has slowed. This paper revives HSP-based algorithm design by examining new group structures with direct relevance to post-quantum cryptography, lattice problems, and structured optimization. We classify a family of finite and continuous groups-extensions and semi-direct products of abelian groups, matrix groups, and certain non-abelian groups-accord...

— Yalla Jnan , Devi Satya Prasad 2026-01-03 00:00:00

Molecular Maxwell Demons as Information Catalysts for Post-Hoc Multi-Condition Mass Spectrometry

We present a theoretical and computational framework for virtual mass spectrometry based on Molecular Maxwell Demons (MMDs) operating as information catalysts. Building on the biological Maxwell demon framework, we demonstrate that mass spectrometry data contain categorical state information that is fundamentally independent of experimental conditions. MMDs implement dual filtering architectures - where the input philtre represents experimental parameters (temperature, collision energy, ionis...

— Kundai, Sachikonye 2025-12-31 00:00:00

Revealing amyloid-β peptide isoforms, including post-translationally modified species, using electrochemical profiling with a dual-electrode set-up

The amyloid-β (Aβ) peptides are crucial biomarkers for the diagnosis of Alzheimer's disease (AD), the most common neurodegenerative disease. The high diversity of the Aβ family provides a significant challenge for recognizing various Aβ forms, which may differ by a single amino acid or a post-translational modification. Such variation at the N-terminus of Aβ peptides leads to changes in their properties associated with typical AD biomolecular mechanisms, such as aggregation or generation...

— Patrycja, Ciosek-Skibińska 2025-12-31 00:00:00

Microenvironment-controlled and red-light-activatable photocage

Photocages have emerged as powerful tools for enabling precise control over the timing, location and quantity of the released biomolecule of interest. However, most commonly used photocages require activation by UV or violet light, which is cytotoxic. Moreover, the exclusive control of light on their photoreactivity, independent of cell microenvironments, leads to off-target biological effects. In this regard, by exploiting the local polarity-regulated excited state of the solvatochromic prob...

— Andrey , Klymchenko 2025-12-31 00:00:00

Anthraquinone-based fluorophore as electrostatic complexation-driven turn-on probe for Cardiolipin detection in model membranes

Cardiolipin (CL), being a mitochondria-specific phospholipid, is known to play a pivotal role in cellular respiration, and its levels are intricately connected to various neurodegenerative disorders. Owing to the simplicity compared to mass spectrometry-based lipidomics, optical detection of CL is an attractive option; however, only two probes, namely TTAPE-Me and 10- nonyl acridine orange (NAO), are currently available. Herein, we report the use of an anthraquinone-based fluorophore 1 as a t...

— Tathagata, Ray 2025-12-30 00:00:00

PMACS: Porous Membrane Adsorption for Capturing Small Extracellular Vesicles Enables Accessible Single-Particle Analysis

Quantitative analysis of small extracellular vesicles (sEVs) at single-particle resolution remains challenging due to their nanoscale dimensions and compositional heterogeneity. Existing methods often rely on specialized and costly instrumentation, limiting accessibility for many researchers and necessitates extensive optimization. Here, we present a porous membrane adsorption for capturing small vesicles (PMACS) which employs a porous anodized aluminum oxide (AAO) membrane as nanoscale compa...

— Seiichi, Nishizawa 2025-12-30 00:00:00

Dried Milk Spots: A viable approach for assessing the chemical exposome in mothers and their infants by targeted LC-MS/MS

Breast milk is a key matrix for assessing early-life exposure. Dried milk spots (DMS) and microsampling devices are convenient low-volume sampling alternatives. Here, a sample preparation protocol and LC-MS/MS method for (semi-)quantitively assessing 216 xenobiotics in DMS were optimized and evaluated. Two extraction solutions were compared. Both approaches performed similarly, with about 50% of analytes falling within the assigned acceptance range for matrix effects (60-140%), and about 80% ...

— Benedikt, Warth 2025-12-29 00:00:00

Spin-Enhanced Electrochemiluminescence from Double-Decker Lanthanide Porphyrin Rotors

Electrochemiluminescence (ECL) is traditionally realized with conformationally rigid luminophores, where suppression of intra-molecular motion is regarded as a prerequisite for efficient emission. Here, we report a fundamentally inverse ECL paradigm ena-bled by lanthanide-bridged double-decker porphyrin molecular rotors, in which electrically stimulated intramolecular rotation amplifies ECL output. Using La3+/Ce4+ bis(tetra(pyridyl)porphyrin) complexes as prototypical systems, we demonstrate ...

— Yingying, Wang 2025-12-29 00:00:00

Thermodynamics-driven synthesis of phase-pure Zr-porphyrin metal–organic framework nanoparticles via auxiliary linker substitution

Metal–organic framework nanoparticles (MOF NPs) are ideal metalloenzymes mimics. Among them, peroxidase-mimicking porphyrin-based MOFs are of interest because of their stability and multifunctionality. However, identical porphyrin linkers and metal nodes like Zr6 nodes form diverse crystal phases, hindering the reliable production and practical applications of phase-pure Zr-porphyrin MOF NPs. Herein, biphenyl-4,4′-dicarboxylic acid with diverse meta-substituents (X-BPDC), including electr...

— Hui, Wei 2025-12-28 00:00:00

Cryogenic Gas-Phase IR Spectroscopy on a Commercial Ion Mobility-Mass Spectrometry Platform

The combination of gas-phase infrared (IR) spectroscopy with ion mobility spectrometry and mass spectrometry provides detailed, multi-dimensional structural information that facilitates the identification of unknown analytes. The instrumentation for performing these measurements is typically home-built and requires substantial expertise to operate. Here, we demonstrate messenger-tagging IR spectroscopy on a modified Synapt G2-S ion mobility-mass spectrometer. Messenger-tagging is performed in...

— Kevin, Pagel 2025-12-28 00:00:00

Browsing FAIR Transformation Product Information with FAIR-TPs

Considering transformation products (TPs) in environmental studies remains a huge challenge for scientists, from identification in samples via mass spectrometry through to inclusion in chemical regulation. This article introduces FAIR-TPs, a website to browse openly-available TP data collated from literature sources. The data is sourced from several community-contributed environmental datasets on the NORMAN Suspect List Exchange (NORMAN-SLE), plus a dataset from ChEMBL available through PubCh...

— Emma, Palm 2025-12-28 00:00:00