Stable isotope standards and capture by anti-peptide antibodies
From Mass Spec Terms
| IUPAC RECOMMENDATIONS 2013 |
| Stable isotope standards and capture by anti-peptide antibodies (SISCAPA) |
|---|
| Method for absolute quantitation of a protein or proteins in a multiplexed fashion using isotope-labeled peptides as surrogate internal standards. The labeled peptides are produced by synthesizing a gene coding for each desired peptide, cell-free transcription, and translation of this gene in a medium containing 13C6-labeled lysine, and digesting with trypsin the expressed protein composed of the desired concatenated peptides. For low-abundance proteins the sensitivity is increased by selective enrichment of the target peptides by antibody capture. |
| Related Term(s): |
| Reference(s):
L. Anderson, C. L. Hunter. Mol. Cell Proteomics 5, 573 (2006). |
| From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013. |
