Label-free quantification
From Mass Spec Terms
| DRAFT DEFINITION |
| Label-free quantification |
|---|
| Method of determining the concentration of proteins through a combination of protein digestion and LC/MS without relying on stable isotope labeling. Protein quantification is achieved by measuring the signal intensity of peptide ions corresponding to a protein or by counting and comparing the number of fragment mass spectra identifying peptides of a given protein. |
| Related Term(s): |
| Reference(s):
M. Bantscheff, M. Schirle, G. Sweetman, J. Rick, B. Kuster, Anal. Bioanal. Chem. 389 (2007) 1017. |
| This is an unofficial draft definition presented for information and comment. |
