Label-free quantification: Difference between revisions
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def=Method of determining the concentration of proteins through a combination of protein digestion and [[LC/MS]] without relying on stable isotope labeling. Protein quantification is achieved by measuring the signal intensity of peptide ions corresponding to a protein or by counting and comparing the number of fragment [[mass spectra]] identifying peptides of a given protein. | def=Method of determining the concentration of proteins through a combination of protein digestion and [[LC/MS]] without relying on stable isotope labeling. Protein quantification is achieved by measuring the signal intensity of peptide ions corresponding to a protein or by counting and comparing the number of fragment [[mass spectra]] identifying peptides of a given protein. | ||
Revision as of 20:58, 4 April 2014
| DRAFT DEFINITION |
| Label-free quantification |
|---|
| Method of determining the concentration of proteins through a combination of protein digestion and LC/MS without relying on stable isotope labeling. Protein quantification is achieved by measuring the signal intensity of peptide ions corresponding to a protein or by counting and comparing the number of fragment mass spectra identifying peptides of a given protein. |
| Related Term(s): |
| Reference(s):
M. Bantscheff, M. Schirle, G. Sweetman, J. Rick, B. Kuster, Anal. Bioanal. Chem. 389 (2007) 1017. |
| This is an unofficial draft definition presented for information and comment. |
